In vivo Phage Display: A promising selection strategy for the improvement of antibody targeting and drug delivery properties.

ABSTRACT: The discovery of hybridoma technology, described by Kohler and Milstein in 1975, and the resulting ability to generate monoclonal antibodies (mAbs) initiated a new era in antibody research and clinical development. However, limitations of the hybridoma technology as a routine antibody generation method in conjunction with high immunogenicity responses have led to the development of alternative approaches for the streamlined identification of most effective antibodies. Within this context, display selection technologies such as phage display, ribosome display, yeast display, bacterial display, and mammalian cell surface display have been widely promoted over the past three decades as ideal alternatives to traditional hybridoma methods. The display of antibodies on phages is probably the mos

SUBMITTER: Andre AS 

PROVIDER: S-EPMC9549074 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature