Circular RNAs in peripheral blood mononuclear cells are more stable than linear RNAs upon sample processing delay.

ABSTRACT: Circular RNAs (circRNAs) are a novel class of RNAs with closed loop structure. Blood circRNAs are widely acknowledged to be more stable than linear mRNAs, which show promising prospect to be liquid biopsy biomarkers for clinical applications. However, accumulating studies have demonstrated that sample processing delays have profound effects on blood transcriptome expression profiles, wherein knowledge remains elusive about the impacts of prolonged sample processing on blood expression profiles of circRNAs. We collected whole blood samples from three donors and isolated peripheral blood mononuclear cells (PBMCs) at six different incubation time points. We measured total RNA expression profiles using RNA sequencing (RNA-seq) and investigated the differentially expressed circRNAs, mRNAs and l

SUBMITTER: Wen G 

PROVIDER: S-EPMC9549506 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature