High level of polarized engraftment of porcine intrahepatic cholangiocyte organoids in decellularized liver scaffolds.

ABSTRACT: In Europe alone, each year 5500 people require a life-saving liver transplantation, but 18% die before receiving one due to the shortage of donor organs. Whole organ engineering, utilizing decellularized liver scaffolds repopulated with autologous cells, is an attractive alternative to increase the pool of available organs for transplantation. The development of this technology is hampered by a lack of a suitable large-animal model representative of the human physiology and a reliable and continuous cell source. We have generated porcine intrahepatic cholangiocyte organoids from adult stem cells and demonstrate that these cultures remained stable over multiple passages whilst retaining the ability to differentiate into hepatocyte- and cholangiocyte-like cells. Recellularization onto porcin

SUBMITTER: Kruger M 

PROVIDER: S-EPMC9549510 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature