Dual control of formin-nucleated actin assembly by the chromatin and ER in mouse oocytes.

ABSTRACT: The first asymmetric meiotic cell divisions in mouse oocytes are driven by formin 2 (FMN2)-nucleated actin polymerization around the spindle. In this study, we investigated how FMN2 is recruited to the spindle peripheral ER and how its activity is regulated in mouse meiosis I (MI) oocytes. We show that this process is regulated by the Ran GTPase, a conserved mediator of chromatin signal, and the ER-associated protein VAPA. FMN2 contains a nuclear localization sequence (NLS) within a domain (SLD) previously shown to be required for FMN2 localization to the spindle periphery. FMN2 NLS is bound to the importin α1/β complex, and the disruption of this interaction by Ran^GTP is required for FMN2 accumulation in the area proximal to the chromatin and the MI spindle. The importin-free F

SUBMITTER: Wang H 

PROVIDER: S-EPMC9549573 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature