Immunological characterization and function analysis of L-type lectin from spotted knifejaw, Oplegnathus punctatus.

ABSTRACT: Lily-type lectin (LTL) plays significant roles in innate immune response against pathogen infection. LTL in animals and plants has received widespread attention. In the present study, an LTL (OppLTL) was identified from spotted knifejaw Oplegnathus punctatus. The OppLTL encoded a typical Ca²⁺-dependent carbohydrate-binding protein containing a CRD domain. The qRT-PCR showed that it was mainly expressed in the gill and was significantly upregulated after Vibrio anguillarum challenge. The agglutination analysis showed that the recombinant OppLTL could bind and agglutinate Gram-negative and Gram-positive bacteria in a Ca²⁺-dependent manner. However, the binding activity was different. Meanwhile, the recombinant OppLTL could hemagglutinate mammalian and tele

SUBMITTER: Liu J 

PROVIDER: S-EPMC9549603 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature