Diversification of phenolic glucosides by two UDP-glucosyltransferases featuring complementary regioselectivity.

ABSTRACT:

Background

Glucoside natural products have been showing great medicinal values and potentials. However, the production of glucosides by plant extraction, chemical synthesis, and traditional biotransformation is insufficient to meet the fast-growing pharmaceutical demands. Microbial synthetic biology offers promising strategies for synthesis and diversification of plant glycosides.

Results

In this study, the two efficient UDP-glucosyltransferases (UGTs) (UGT85A1 and RrUGT3) of plant origin, that are capable of recognizing phenolic aglycons, are characterized in vitro. The two UGTs show complementary regioselectivity towards the alcoholic and phenolic hydroxyl groups on phenolic substrates. By combining a developed alkylphenol bio-oxidation system and these UGTs, twenty-four p

SUBMITTER: Guo F 

PROVIDER: S-EPMC9549646 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature