Label-free Super-resolution Imaging Enabled by Vibrational Imaging of Swelled Tissue and Analysis.

ABSTRACT: The universal utilization of fluorescence microscopy, especially super-resolution microscopy, has greatly advanced knowledge about modern biology. Conversely, the requirement of fluorophore labeling in fluorescent techniques poses significant challenges, such as photobleaching and non-uniform labeling of fluorescent probes and prolonged sample processing. In this protocol, the detailed working procedures of vibrational imaging of swelled tissue and analysis (VISTA) are presented. VISTA circumvents obstacles associated with fluorophores and achieves label-free super-resolution volumetric imaging in biological samples with spatial resolution down to 78 nm. The procedure is established by embedding cells and tissues in hydrogel, isotropically expanding the hydrogel sample hybrid, and visualiz

SUBMITTER: Miao K 

PROVIDER: S-EPMC9549918 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature