In vitro osteoblast activity is decreased by residues of chemicals used in the cleaning and viral inactivation process of bone allografts.

ABSTRACT: Allograft bone tissue has a long history of use. There are two main ways of preserving allografts: by cold (freezing), or at room temperature after an additional cleaning treatment using chemicals. These chemicals are considered potentially harmful to humans. The aim of the study was (i) to assess the presence of chemical residues on processed bone allografts and (ii) to compare the in vitro biocompatibility of such allografts with that of frozen allografts. The presence of chemical residues on industrially chemically treated bone was assessed by high performance liquid chromatography (HPLC) after extraction. Biocompatibility analysis was performed on primary osteoblast cultures from Wistar rats grown on bone disks, either frozen (F-bone group) or treated with supercritical carbon dioxide

SUBMITTER: Villatte G 

PROVIDER: S-EPMC9550034 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature