Solution NMR Structure of Titin N2A Region Ig Domain I83 and Its Interaction with Metal Ions.

ABSTRACT: Titin, the largest single chain protein known so far, has long been known to play a critical role in passive muscle function but recent studies have highlighted titin's role in active muscle function. One of the key elements in this role is the Ca²⁺-dependent interaction between titin's N2A region and the thin filament. An important element in this interaction is I83, the terminal immunoglobulin domain in the N2A region. There is limited structural information about this domain, but experimental evidence suggests that it plays a critical role in the N2A-actin binding interaction. We now report the solution NMR structure of I83 and characterize its dynamics and metal binding properties in detail. Its structure shows interesting relationships to other I-band Ig domains. Metal bind

SUBMITTER: Kelly C 

PROVIDER: S-EPMC9550216 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature