Fasudil inhibits hepatic artery spasm by repressing the YAP/ERK/ ET_A/ET_B signaling pathway via inhibiting ROCK activation.

ABSTRACT:

Objective

To explore the effect of Fasudil on HA spasm and its underlying mechanism.

Methods

Rabbits were divided into Sham, Fasudil, and Model groups for experiments. Fasudil was injected into the left medial lobe of the rabbit liver using a 16G lumbar puncture needle through the laparotomic route. The spasm model was established by inserting the catheter sheath into the femoral arteries of rabbits, followed by celiac artery angiography and left HA catheterization with a micro-catheter. Next, the GSE60887 and GSE37924 datasets concerning Fasudil treatment were analyzed. Moreover, immunofluorescence staining was conducted for YAP1 and α-SMA. Finally, Western blotting was performed to examine the expressions of YAP1, ROCK, ERK1/2, ET_A, and ET_B.

Result

SUBMITTER: Hao X 

PROVIDER: S-EPMC9550242 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature