VEGF-A promotes the motility of human melanoma cells through the VEGFR1-PI3K/Akt signaling pathway.

ABSTRACT: Vascular endothelial growth factor A (VEGF-A) and its receptors (VEGFR1 and R2) play important roles in the progression of malignant melanoma through tumor angiogenesis. However, it is not clear whether the VEGF-A/VEGFR1 signaling pathway is involved in the proliferation and migration of melanoma cells. Thus, the effect of VEGF-A on cell migration was investigated in human melanoma cell lines. Of several splicing variants of VEGF-A, VEGF₁₆₅ is the most abundant and responsible for VEGF-A biological potency. VEGF₁₆₅ facilitated the migration of melanoma cells in both a chemotactic and chemokinetic manner, but cell proliferation was not affected by VEGF₁₆₅. VEGF₁₆₅ also induced the phosphorylation of Akt. In addition, VEGF₁₆₅-induced cell migration was inhibited significantly by VEGFR1/2 or a VEGFR1-neutralizing antibody. Furthermore, the downregulation of VEGFR1 via the transfection of VEGFR1-targeting antisense oligonucleotides suppressed VEGF₁₆₅-induced cell migration. Moreover, wortmannin, an inhibitor of phosphatidylinositol-3 kinase (PI3K) in the PI3K/Akt pathway, suppressed VEGF₁₆₅-induced Akt phosphorylation and VEGF₁₆₅-induced cell migration. These findings suggest that the motility of melanoma cells is regulated by signals mediated through the PI3K/Akt kinase pathway with the activation of VEGFR1 tyrosine kinase by VEGF₁₆₅. Thus, the downregulation of signaling via VEGF-A/VEGFR1 might be an effective therapeutic approach that could prevent the progression of malignant melanoma.

SUBMITTER: Koizumi K 

PROVIDER: S-EPMC9550759 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature