Allele-specific differential regulation of monoallelically expressed autosomal genes in the cardiac lineage.

ABSTRACT: Each mammalian autosomal gene is represented by two alleles in diploid cells. To our knowledge, no insights have been made in regard to allele-specific regulatory mechanisms of autosomes. Here we use allele-specific single cell transcriptomic analysis to elucidate the establishment of monoallelic gene expression in the cardiac lineage. We find that monoallelically expressed autosomal genes in mESCs and mouse blastocyst cells are differentially regulated based on the genetic background of the parental alleles. However, the genetic background of the allele does not affect the establishment of monoallelic genes in differentiated cardiomyocytes. Additionally, we observe epigenetic differences between deterministic and random autosomal monoallelic genes. Moreover, we also find a greater contrib

SUBMITTER: Balasooriya GI 

PROVIDER: S-EPMC9550772 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature