Developing a multiplex PCR-based assay kit for bloodstream infection by analyzing genomic big data.

ABSTRACT:

Background

In recent years, the incidence of bloodstream infections (BSI) has increased, the composition of pathogenic bacteria has changed, and drug resistance among bacteria has gradually increased due to the widespread use of interventional techniques, broad-spectrum antibacterial drugs, hormones, and immunosuppressive agents. Here, we have developed a multiplex PCR assay kit for the detection of pathogens (14 Gram-negative bacteria, 15 Gram-positive bacteria, and 4 fungi) in whole blood from patients with BSI using five-color fluorescent multiplex PCR followed by capillary electrophoresis. Our assay exhibits a diagnosis of higher quality and an improved detection rate for common pathogens.

Methods

A local genome DNA database of 33 pathogenic bacteria was constructed. Nex

SUBMITTER: Zhang D 

PROVIDER: S-EPMC9550966 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature