Validation of high concentrated thrombin time assay for unfractionated heparin monitoring.

ABSTRACT:

Background

The high concentrated thrombin time (hcTT), a thrombin time modified by increasing the thrombin concentration, is a possible alternative assay to activated partial thromboplastin time (aPTT) in unfractionated heparin (UFH) monitoring. This study aimed to determine the optimal thrombin concentration used in the hcTT assay for UFH monitoring.

Methods

A total of 30 blood samples obtained from healthy volunteers were included in this study. Thrombin concentrations of 10.0, 15.0, 20.0, and 25.0 IU/ml were used in the hcTT assay. The consistency between the hcTT and anti-FXa assays was evaluated. To validate the hcTT assay, linearity, repeatability, reproducibility, and diagnostic performance of the assay were assessed.

Results

The hcTT assay using thrombin conce

SUBMITTER: Apipongrat D 

PROVIDER: S-EPMC9550976 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature