Ornithine aminotransferase and carbamoyl phosphate synthetase 1 involved in ammonia metabolism serve as novel targets for early stages of gastric cancer.

ABSTRACT:

Objective

The sensitivity and specificity of current biomarkers for gastric cancer were insufficient. The aim of the present study was to screen novel biomarkers and determine the diagnostic values of ornithine aminotransferase (OAT) and carbamoyl phosphate synthetase 1 (CPS1) for detecting gastric cancer.

Methods

With stable isotope tags, we labelled an initial discovery group of four paired gastric cancer tissue samples and identified with LC-ESI-MS/MS. A validation group of 159 gastric cancer samples and 30 healthy controls were used to validate the candidate targets. GSEA was used to explore the pathways activated in gastric cancer.

Results

Four hundred and thirty one proteins were found differentially expressed in gastric cancer tissues. Of these proteins, OAT an

SUBMITTER: Jiang Z 

PROVIDER: S-EPMC9551119 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature