ICAT promotes colorectal cancer metastasis via binding to JUP and activating the NF-κB signaling pathway.

ABSTRACT:

Background

The inhibitor of β-catenin and T-cell factor (ICAT) is a direct negative regulator of the canonical Wnt signaling pathway, which is an attractive therapeutic target for colorectal cancer (CRC). Accumulating evidence suggests that ICAT interacts with other proteins to exert additional functions, which are not yet fully elucidated.

Methods

The overexpression of ICAT of CRC cells was conducted by lentivirus infection and plasmids transfection and verified by quantitative real-time reverse transcription-polymerase chain reaction (real-time RT-PCR) and Western blotting. The effect of ICAT on the mobility of CRC cells was assessed by wound healing assay and transwell assay in vitro and lung metastasis in vivo. New candidate ICAT-interacting proteins were explored and ve

SUBMITTER: Wang Z 

PROVIDER: S-EPMC9551128 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature