Molecular characterization of aviadenovirus serotypes and pathogenicity of the identified adenovirus in broiler chickens.

ABSTRACT: Inclusion body hepatitis (IBH) is an economically significant viral disease that primarily affects broiler chickens. At least 12 different aviadenovirus serotypes are responsible for causing IBH. This study aimed to use polymerase chain reaction (PCR) and phylogenetic analysis to characterize fowl adenovirus isolates that were in circulation from 2019 to 2021 and investigate the pathogenicity of the isolated strains in commercial broiler chickens. Suspected liver samples were molecularly identified using hexon gene targeting by PCR, and viruses were isolated using chick embryo liver cell culture. For serotype identification, the fowl adenovirus-positive samples were subjected to hexon gene sequencing and phylogenetic analysis. The pathogenicity of two isolates was tested in commercial chic

SUBMITTER: Lebdah M 

PROVIDER: S-EPMC9551142 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature