Single-cell transcriptomics reveals the role of Macrophage-Naive CD4 + T cell interaction in the immunosuppressive microenvironment of primary liver carcinoma.

ABSTRACT:

Background

Liver carcinoma generally presents as an immunosuppressive microenvironment that promotes tumor evasion. The intercellular crosstalk of immune cells significantly influences the construction of an immunosuppressive microenvironment. This study aimed to investigate the important interactions between immune cells and their targeting drugs in liver carcinoma, by using single-cell and bulk transcriptomic data.

Methods

Single-cell and bulk transcriptomic data were retrieved from Gene Expression Omnibus (GSE159977, GSE136103, and GSE125449) and The Cancer Genome Atlas (TGCA-LIHC), respectively. Quality control, dimension reduction, clustering, and annotation were performed according to the Scanpy workflow based on Python. Cell-cell interactions were explored using the C

SUBMITTER: Mo Z 

PROVIDER: S-EPMC9552358 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature