Engineering sigma factors and chaperones for enhanced heterologous lipoxygenase production in Escherichia coli.

ABSTRACT:

Background

Lipoxygenase (EC. 1.13.11.12, LOX) can catalyze the addition of oxygen into polyunsaturated fatty acids to produce hydroperoxides, which are widely used in the food, chemical, and pharmaceutical industries. In recent years, the heterologous production of LOX by Escherichia coli has attracted extensive attention. However, overexpressed recombinant LOX in E. coli aggregates and forms insoluble inclusion bodies owing to protein misfolding.

Results

In this study, a split green fluorescent protein-based screening method was developed to screen sigma (σ) factors and molecular chaperones for soluble LOX expression. Three mutant libraries of Skp, GroES, and RpoH was analyzed using the high-throughput screening method developed herein, and a series of mutants with signific

SUBMITTER: Pang C 

PROVIDER: S-EPMC9552429 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature