<i>Cryptococcus neoformans</i> releases proteins during intracellular residence that affect the outcome of the fungal-macrophage interaction.

ABSTRACT: Cryptococcus neoformans is a facultative intracellular pathogen that can replicate and disseminate in mammalian macrophages. In this study, we analyzed fungal proteins identified in murine macrophage-like cells after infection with C. neoformans. To accomplish this, we developed a protocol to identify proteins released from cryptococcal cells inside macrophage-like cells; we identified 127 proteins of fungal origin in infected macrophage-like cells. Among the proteins identified was urease, a known virulence factor, and others such as transaldolase and phospholipase D, which have catalytic activities that could contribute to virulence. This method provides a straightforward methodology to study host-pathogen interactions. We chose to study further Yeast Oligomycin Resistance

SUBMITTER: Jung EH 

PROVIDER: S-EPMC9552768 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature