Quantitative proteomics of small numbers of closely-related cells: Selection of the optimal method for a clinical setting.

ABSTRACT: Mass spectrometry (MS)-based proteomics profiling has undoubtedly increased the knowledge about cellular processes and functions. However, its applicability for paucicellular sample analyses is currently limited. Although new approaches have been developed for single-cell studies, most of them have not (yet) been standardized and/or require highly specific (often home-built) devices, thereby limiting their broad implementation, particularly in non-specialized settings. To select an optimal MS-oriented proteomics approach applicable in translational research and clinical settings, we assessed 10 different sample preparation procedures in paucicellular samples of closely-related cell types. Particularly, five cell lysis protocols using different chemistries and mechanical forces were combine

SUBMITTER: van der Pan K 

PROVIDER: S-EPMC9553008 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature