Whole Transcriptomic Analysis of Key Genes and Signaling Pathways in Endogenous ARDS.

ABSTRACT:

Objective

To analyze the differentially expressed genes (DEGs) in rats with endogenous acute respiratory distress syndrome (ARDS) lung injury and explore the pathogenesis and early diagnostic molecular markers using whole transcriptomic data.

Methods

Twelve 8-week-old male Sprague Dawley rats were selected and randomly and equally divided into ARDS lung injury group and normal control group. RNA was extracted from the left lung tissues of both the groups and sequenced using the paired-end sequencing mode of the Illumina Hiseq sequencing platform. The DEGs of miRNA, cirRNA, lncRNA, and mRNA were screened using DESeq2 software, and the ceRNA regulatory network was constructed using Cytoscape. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment anal

SUBMITTER: Xie Y 

PROVIDER: S-EPMC9553538 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature