Somatic embryogenesis and plant regeneration from transverse thin cell layers of adult peach palm (Bactris gasipaes) lateral offshoots.

ABSTRACT: In this work, we report a successful protocol to obtain in vitro peach palm (Bactris gasipaes Kunth) "Diamantes 10" plants through somatic embryogenesis from transverse thin cell layer (TCL) explants, dissected from three sections (basal, medial, and apical) of lateral offshoots of adult plants cultured on different concentrations of 4-amino-3,5,6-trichloropicolonic acid (picloram). After swelling and development of primary callus in all treatments, without any strong effect of explant origin or picloram concentration, it was possible to observe the formation of embryogenic structures and the exact point from where they developed. Browning was also observed and correlated to the induction treatments, although it was not an impairment for the production of embryogenic structur

SUBMITTER: Campos-Boza S 

PROVIDER: S-EPMC9554471 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature