Effect of amino acids on transcription and translation of key genes in E. coli K and B grown at a steady state in minimal medium.

ABSTRACT: Growing E. coli to high densities is a common strategy for biologicals production. The process is implemented by using complex or minimal media with different feeding strategies. To understand the effect of amino acids, E. coli B and K were grown at a steady state of 0.35 h^-1 in glucose minimal medium with and without amino acids, while their metabolism, protein abundance and gene expression were compared. The results showed that amino acids promoted higher acetate excretion, higher fatty acid biosynthesis (K strain), repressed glucose uptake rate, and decreased expression of proteins associated with the TCA cycle, glyoxylate shunt and amino acid biosynthesis. In presence of amino acids, E. coli K upregulated fatty acid biosynthesis and repressed more genes and proteins involved

SUBMITTER: Baez A 

PROVIDER: S-EPMC9554925 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature