Surface fungal diversity and several mycotoxin-related genes' expression profiles during the Lunar Palace 365 experiment.

ABSTRACT:

Background

Chinese Lunar Palace 1 (LP1) is a ground-based bio-regenerative life support system (BLSS) test bed integrating highly efficient plant cultivation, animal protein production, urine nitrogen recycling, and bioconversion of solid waste. To date, there has been no molecular method-based detailed investigation of the fungal community and mycotoxin potential in BLSS habitats. To ensure safe BLSS design for actual space missions, we analyzed the LP1 surface mycobiome and mycotoxin potential during the Lunar Palace 365 project through internal transcribed spacer region 1 (ITS1) amplicon sequencing and quantitative polymerase chain reaction (qPCR) with primers specific for idh, ver1, nor1, tri5, and ITS1.

Results

The LP1 system exhibited significant differences in fungal

SUBMITTER: Yang J 

PROVIDER: S-EPMC9555122 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature