Fabrication of composite microfluidic devices for local control of oxygen tension in cell cultures.

ABSTRACT: Oxygen tension is a central component of the cellular microenvironment and can serve as a trigger for changes in cell phenotype and function. There is a strong need to precisely control and modulate oxygen tension in cell culture systems in order to more accurately model the physiology and pathophysiology observed in vivo. The objective of this paper was to develop a simple, yet effective strategy for local control of oxygen tension in microfluidic cell cultures. Our strategy relied on fabrication of microfluidic devices using oxygen-permeable and impermeable materials. This composite device was designed so as to incorporate regions of gas permeability into the roof of the cell culture chamber and was outfitted with a reservoir for the oxygen-consuming chemical pyrogallol. When assembled a

SUBMITTER: Gao Y 

PROVIDER: S-EPMC9555225 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature