Epitope vaccine design for Toxoplasma gondii based on a genome-wide database of membrane proteins.

ABSTRACT:

Background

There is presently no effective and safe vaccine for Toxoplasma gondii for humans. The study described here was designed to search for a novel group of optimal B cell and T cell epitopes from Toxoplasma membrane proteins using genome-wide comprehensive screening.

Methods

The amino acid sequences of membrane proteins of T. gondii were obtained from the UniProt database. The ABCPred and BepiPred servers were employed to predict the linear B cell epitopes. The Immune Epitope Database (IEDB) online service was utilized to forecast T cell epitopes within T. gondii membrane proteins that bind to human leukocyte antigen (HLA) class I (HLA-I) or HLA-II molecules.

Results

From the 314 membrane proteins of T. gondii, a total of 14 linear B cell epitopes embedded in 1

SUBMITTER: Li XW 

PROVIDER: S-EPMC9555269 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature