Termination of Ca²⁺ puffs during IP₃-evoked global Ca²⁺ signals.

ABSTRACT: We previously described that cell-wide cytosolic Ca²⁺ transients evoked by inositol trisphosphate (IP₃) are generated by two modes of Ca²⁺ liberation from the ER; 'punctate' release via an initial flurry of transient Ca²⁺ puffs from local clusters of IP₃ receptors, succeeded by a spatially and temporally 'diffuse' Ca²⁺ liberation. Those findings were derived using statistical fluctuation analysis to monitor puff activity which is otherwise masked as global Ca²⁺ levels rise. Here, we devised imaging approaches to resolve individual puffs during global Ca²⁺ elevations to better investigate the mechanisms terminating the puff flurry. We find that puffs contribute about 40% (∼90 attomoles) of the total Ca

SUBMITTER: Lock JT 

PROVIDER: S-EPMC9555318 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature