Genomic characterization of Francisella tularensis and other diverse Francisella species from complex samples.

ABSTRACT: Francisella tularensis, the bacterium that causes the zoonosis tularemia, and its genetic near neighbor species, can be difficult or impossible to cultivate from complex samples. Thus, there is a lack of genomic information for these species that has, among other things, limited the development of robust detection assays for F. tularensis that are both specific and sensitive. The objective of this study was to develop and validate approaches to capture, enrich, sequence, and analyze Francisella DNA present in DNA extracts generated from complex samples. RNA capture probes were designed based upon the known pan genome of F. tularensis and other diverse species in the family Francisellaceae. Probes that targeted genomic regions also present in non-Francisellaceae species were excluded, and p

SUBMITTER: Wagner DM 

PROVIDER: S-EPMC9555625 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature