Maturation and circuit integration of transplanted human cortical organoids.

ABSTRACT: Self-organizing neural organoids represent a promising in vitro platform with which to model human development and disease^1-5. However, organoids lack the connectivity that exists in vivo, which limits maturation and makes integration with other circuits that control behaviour impossible. Here we show that human stem cell-derived cortical organoids transplanted into the somatosensory cortex of newborn athymic rats develop mature cell types that integrate into sensory and motivation-related circuits. MRI reveals post-transplantation organoid growth across multiple stem cell lines and animals, whereas single-nucleus profiling shows progression of corticogenesis and the emergence of activity-dependent transcriptional programs. Indeed, transplanted cortical neurons display more comp

SUBMITTER: Revah O 

PROVIDER: S-EPMC9556304 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature