Shisa7 phosphorylation regulates GABAergic transmission and neurodevelopmental behaviors.

ABSTRACT: GABA-A receptors (GABA_ARs) are crucial for development and function of the brain. Altered GABAergic transmission is hypothesized to be involved in neurodevelopmental disorders. Recently, we identified Shisa7 as a GABA_AR auxiliary subunit that modulates GABA_AR trafficking and GABAergic transmission. However, the underlying molecular mechanisms remain elusive. Here we generated a knock-in (KI) mouse line that is phospho-deficient at a phosphorylation site in Shisa7 (S405) and combined with electrophysiology, imaging and behavioral assays to illustrate the role of this site in GABAergic transmission and plasticity as well as behaviors. We found that expression of phospho-deficient mutants diminished α2-GABA_AR trafficking in heterologous cells. Addit

SUBMITTER: Wu K 

PROVIDER: S-EPMC9556544 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature