Ontology highlight
ABSTRACT:
SUBMITTER: Novakova Z
PROVIDER: S-EPMC9556554 | biostudies-literature | 2022 Oct
REPOSITORIES: biostudies-literature
Novakova Zora Z Milosevic Mirko M Kutil Zsofia Z Ondrakova Marketa M Havlinova Barbora B Kasparek Petr P Sandoval-Acuña Cristian C Korandova Zuzana Z Truksa Jaroslav J Vrbacky Marek M Rohlena Jakub J Barinka Cyril C
Scientific reports 20221012 1
In humans, disruptions in the heme biosynthetic pathway are associated with various types of porphyrias, including variegate porphyria that results from the decreased activity of protoporphyrinogen oxidase IX (PPO; E.C.1.3.3.4), the enzyme catalyzing the penultimate step of the heme biosynthesis. Here we report the generation and characterization of human cell lines, in which PPO was inactivated using the CRISPR/Cas9 system. The PPO knock-out (PPO-KO) cell lines are viable with the normal prolif ...[more]