Effects of molecular weight on intestinal anti-inflammatory activities of β-D-glucan from Ganoderma lucidum.

ABSTRACT: To investigate the influence of molecular weight (M _w) on the anti-inflammatory activity of β-D-glucan from Ganoderma lucidum, ultrasonic irradiation was applied to treat the β-D-glucan (GLP, 2.42 × 10⁶ g/mol) solution to obtain two degraded fractions with molecular weight of 6.53 × 10⁵ g/mol (GLPC) and 3.49 × 10⁴ g/mol (GLPN). Structural analysis proved that the degraded fractions possessed similar repeated units with the original β-D-glucan. The in vitro anti-inflammatory activity studies showed that all fractions could significantly inhibit LPS-induced expression of cytokines including TNF-α, IL-8, MIF and MCP-1 in Caco-2 cells at certain concentrations. Moreover, GLPC and GLPN exhibited better anti-inflammatory activity t

SUBMITTER: Liu Y 

PROVIDER: S-EPMC9557179 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature