Whole-brain optical access in a small adult vertebrate with two- and three-photon microscopy.

ABSTRACT: Although optical microscopy has allowed scientists to study the entire brain in early developmental stages, access to the brains of live, adult vertebrates has been limited. Danionella, a genus of miniature, transparent fish closely related to zebrafish has been introduced as a neuroscience model to study the adult vertebrate brain. However, the extent of optically accessible depth in these animals has not been quantitatively characterized. Here, we show that both two- and three-photon microscopy can access the entire depth and rostral-caudal extent of the adult wildtype Danionella dracula brain without any modifications to the animal other than mechanical stabilization. Three-photon microscopy provides higher signal-to-background ratio and optical sectioning of fluorescently

SUBMITTER: Akbari N 

PROVIDER: S-EPMC9557827 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature