Effect of CD44 signal axis in the gain of mesenchymal stem cell surface antigens from synovial fibroblasts in vitro.

ABSTRACT: Tissue-residing mesenchymal stromal/stem cells (MSCs) have multipotent characteristics that are important for adult tissue homeostasis and tissue regeneration after injury. We previously reported that fibroblastic cells isolated from the synovial membrane in the knee joint give rise to cells with MSC characteristics in a two-dimensional culture. To explore the molecular mechanisms underlying these hyperplastic properties, we performed time-course surface antigen expression analyses during in vitro culture. Cells freshly isolated from the synovial membrane rarely contained cells that met the criteria (CD45^-CD73⁺CD90⁺CD105⁺). However, the number of cells expressing MSC antigens increased on day 7. Flow cytometric analysis indicated that cell

SUBMITTER: Isono M 

PROVIDER: S-EPMC9557910 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature