High-quality reference transcriptome construction improves RNA-seq quantification in Oryza sativa indica.

ABSTRACT: The Reference Transcriptomic Dataset (RTD) is an accurate and comprehensive collection of transcripts originating from a given organism. It holds the key to precise transcript quantification and downstream analysis of differential expressions and regulations. Currently, transcriptome annotations for most crop plants are far from complete. For example, Oryza sativa indica (O. sativa indica) is reported to have 40,759 transcripts in the Ensembl database without alternative transcript isoforms and alternative splicing (AS) events. To generate a high-quality RTD, we conducted RNA sequencing of rice leaf samples collected at various time points during Rhizoctonia solani infection. The obtained reads were analyzed by adopting the recently developed computational analy

SUBMITTER: Srikakulam N 

PROVIDER: S-EPMC9558114 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature