Transcription profiling-based identification of Staphylococcus aureus genes regulated by the agr and/or sarA loci.

ABSTRACT: The advent of transcription profiling technologies has provided researchers with an unprecedented ability to study biological processes. Accordingly, a custom-made Affymetrix GeneChip, constituting >86% of the Staphylococcus aureus genome, was used to identify open reading frames that are regulated by agr and/or SarA, the two best-studied regulators of the organism's virulence response. RNA extracted from wild-type cells and agr, sarA, and agr sarA mutant cells in the early-, mid-, and late-log and stationary phases of growth was analyzed. Open reading frames with transcription patterns expected of genes either up- or downregulated in an agr- and/or SarA-dependent manner were identified. Oligonucleotide microarray and Northern blot analyses confirmed that the transcription of several known

SUBMITTER: Dunman PM 

PROVIDER: S-EPMC95583 | biostudies-literature | 2001 Dec

REPOSITORIES: biostudies-literature