Ultrabright Foster Resonance Energy Transfer Nanovesicles: The Role of Dye Diffusion.

ABSTRACT: The development of contrast agents based on fluorescent nanoparticles with high brightness and stability is a key factor to improve the resolution and signal-to-noise ratio of current fluorescence imaging techniques. However, the design of bright fluorescent nanoparticles remains challenging due to fluorescence self-quenching at high concentrations. Developing bright nanoparticles showing FRET emission adds several advantages to the system, including an amplified Stokes shift, the possibility of ratiometric measurements, and of verifying the nanoparticle stability. Herein, we have developed Förster resonance energy transfer (FRET)-based nanovesicles at different dye loadings and investigated them through complementary experimental techniques, including conventional fluorescence spectroscop

SUBMITTER: Morla-Folch J 

PROVIDER: S-EPMC9558306 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature