Secretome of atrial epicardial adipose tissue facilitates reentrant arrhythmias by myocardial remodeling.

ABSTRACT:

Background

Epicardial adipose tissue (EAT) accumulation is associated with cardiac arrhythmias. The effect of EAT secretome (EATs) on cardiac electrophysiology remains largely unknown.

Objective

The purpose of this study was to investigate the arrhythmogenicity of EATs and its underlying molecular and electrophysiological mechanisms.

Methods

We collected atrial EAT and subcutaneous adipose tissue (SAT) from 30 patients with atrial fibrillation (AF), and EAT from 3 donors without AF. The secretome was collected after a 24-hour incubation of the adipose tissue explants. We cultured neonatal rat ventricular myocytes (NRVMs) with EATs, subcutaneous adipose tissue secretome (SATs), and cardiomyocytes conditioned medium (CCM) for 72 hours. We implemented the electrophysiolo

SUBMITTER: Ernault AC 

PROVIDER: S-EPMC9558493 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature