Alicyclobacillus curvatus sp. nov. and Alicyclobacillus mengziensis sp. nov., two acidophilic bacteria isolated from acid mine drainage.

ABSTRACT: Two acidophilic strains, designated as ALEF1^T and S30H14^T, were isolated from acid mine drainage sediment. Cells of both strains were Gram-stain-positive, aerobic, endospore-forming rods. Strains ALEF1^T and S30H14^T were acidophilic and mesophilic, the former grew at 20-40 °C (optimum, 30 °C) and pH 2.5-4.5 (optimum, pH 3.5), while the latter grew at 20-45 °C (optimum, 30 °C) and pH 2.0-5.5 (optimum, pH 4.5). The 16S rRNA gene-based sequence analysis revealed that strains ALEF1^T and S30H14^T belonged to the genus Alicyclobacillus, and were phylogenetically close to Alicyclobacillus ferrooxydans TC-34^T with 97.1 and 97.4% similarity, respectively. The similarity between the two novel strains was 98.6 %.

SUBMITTER: Jiang Z 

PROVIDER: S-EPMC9558579 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature