Expanding the genetic engineering toolbox for the metabolically flexible acetogen Eubacterium limosum.

ABSTRACT: Acetogenic bacteria are an increasingly popular choice for producing fuels and chemicals from single carbon (C1) substrates. Eubacterium limosum is a promising acetogen with several native advantages, including the ability to catabolize a wide repertoire of C1 feedstocks and the ability to grow well on agar plates. However, despite its promise as a strain for synthetic biology and metabolic engineering, there are insufficient engineering tools and molecular biology knowledge to leverage its native strengths for these applications. To capitalize on the natural advantages of this organism, here we extended its limited engineering toolbox. We evaluated the copy number of three common plasmid origins of replication and devised a method of controlling copy number and heterologous gene expressio

SUBMITTER: Sanford PA 

PROVIDER: S-EPMC9559302 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature