Dataset Information

Transmission and pathogenicity of canine H3N2 influenza virus in dog and guinea pig models.

ABSTRACT:

Background

Influenza A virus causes respiratory disease in many animal species as well as in humans. Due to the high human-animal interface, the monitoring of canine influenza in dogs and the study of the transmission and pathogenicity of canine influenza in animals are important.

Methods

Eight-week-old beagle dogs (Canis lupus familaris) (n = 13) were used for the intraspecies transmission model. The dogs were inoculated intranasally with 1 ml of 10⁶ EID₅₀ per ml of canine H3N2 influenza virus (A/canine/Thailand/CU-DC5299/2012) (CIV-H3N2). In addition, 4-week-old guinea pigs (Cavia porcellus) (n = 20) were used for the interspecies transmission model. The guinea pigs were inoculated intranasally with 300 µl of 10⁶ EID₅₀ per ml of CIV-H3N2.

Results

For the Thai CIV-H3N2 challenged in the dog model, the incoculated and direct contact dogs developed respiratory signs at 2 dpi. The dogs shed the virus in the respiratory tract at 1 dpi and developed an H3-specific antibody against the virus at 10 dpi. Lung congestion and histopathological changes in the lung were observed. For the Thai CIV-H3N2 challenge in the guinea pig model, the incoculated, direct contact and aerosol-exposed guinea pigs developed fever at 1-2 dpi. The guinea pigs shed virus in the respiratory tract at 2 dpi and developed an H3-specific antibody against the virus at 7 dpi. Mild histopathological changes in the lung were observed.

Conclusion

The result of this study demonstrated evidence of intraspecies and interspecies transmission of CIV-H3N2 in a mammalian model.

SUBMITTER: Tangwangvivat R

PROVIDER: S-EPMC9559841 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature

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Publications

Transmission and pathogenicity of canine H3N2 influenza virus in dog and guinea pig models.

Tangwangvivat Ratanaporn R Chaiyawong Supassama S Nonthabenjawan Nutthawan N Charoenkul Kamonpan K Janethanakit Taveesak T Udom Kitikhun K Kesdangsakonwut Sawang S Tantilertcharoen Rachod R Thontiravong Aunyaratana A Amonsin Alongkorn A

Virology journal 20221012 1

<h4>Background</h4>Influenza A virus causes respiratory disease in many animal species as well as in humans. Due to the high human-animal interface, the monitoring of canine influenza in dogs and the study of the transmission and pathogenicity of canine influenza in animals are important.<h4>Methods</h4>Eight-week-old beagle dogs (Canis lupus familaris) (n = 13) were used for the intraspecies transmission model. The dogs were inoculated intranasally with 1 ml of 10<sup>6</sup> EID<sub>50</sub> p ...[more]

PMID: 36224594

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Dataset Information

Transmission and pathogenicity of canine H3N2 influenza virus in dog and guinea pig models.

Background

Methods

Results

Conclusion

Publications

Transmission and pathogenicity of canine H3N2 influenza virus in dog and guinea pig models.

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