Blocking P2X7 by intracerebroventricular injection of P2X7-specific nanobodies reduces stroke lesions.

ABSTRACT:

Background

Previous studies have demonstrated that purinergic receptors could be therapeutic targets to modulate the inflammatory response in multiple models of brain diseases. However, tools for the selective and efficient targeting of these receptors are lacking. The development of new P2X7-specific nanobodies (nbs) has enabled us to effectively block the P2X7 channel.

Methods

Temporary middle cerebral artery occlusion (tMCAO) in wild-type (wt) and P2X7 transgenic (tg) mice was used to model ischemic stroke. Adenosine triphosphate (ATP) release was assessed in transgenic ATP sensor mice. Stroke size was measured after P2X7-specific nbs were injected intravenously (iv) and intracerebroventricularly (icv) directly before tMCAO surgery. In vitro cultured microglia were used t

SUBMITTER: Wilmes M 

PROVIDER: S-EPMC9559872 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature