Gene delivery to neurons in the auditory brainstem of barn owls using standard recombinant adeno-associated virus vectors.

ABSTRACT: Recombinant adeno-associated virus (rAAV) vectors are a commonly used tool for gene delivery. There is a large choice of different serotypes whose transduction efficiency varies for different animal species. In this study, three rAAV vectors were tested for transduction efficiency in the auditory brainstem of adult barn owls (Tyto alba) which are not standard laboratory animals. Injections with rAAV serotypes 2/1 and 2/5 resulted in reliable expression in various nuclei of the auditory brainstem of barn owls. Both vectors showed evidence of being spread by axonal transport. However, only rAAV2/5 also showed expression in regions far distant from the injection site, suggesting long-range axonal transport in connections along the auditory pathway. In contrast, injections with rAAV2/9

SUBMITTER: Thiele N 

PROVIDER: S-EPMC9559881 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature