Trans-cardiac perfusion of neonatal mice and immunofluorescence of the whole body as a method to study nervous system development.

ABSTRACT: Whole animal perfusion is a well-established method that has been used for the past decades in multiple research fields. Particularly, it has been very important for the study of the brain. The rapid and uniform fixation of tissue is essential for the preservation of its integrity and the study of complex structures. For small tissue pieces submerging in formaldehyde solution oftentimes is sufficient to get a good fixation, larger tissues or organs with a more complicated structure present a greater difficulty. Here, we report the precise parameters to successfully perform trans-cardiac perfusion of neonatal mouse pups that allows a uniform fixation of the whole body for subsequent structural analysis and immunohistochemistry. In comparison to standard perfusion procedures of adult mice, c

SUBMITTER: Perez Arevalo A 

PROVIDER: S-EPMC9560478 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature