Using colony size to measure fitness in Saccharomyces cerevisiae.

ABSTRACT: Competitive fitness assays in liquid culture have been a mainstay for characterizing experimental evolution of microbial populations. Growth of microbial strains has also been extensively characterized by colony size and could serve as a useful alternative if translated to per generation measurements of relative fitness. To examine fitness based on colony size, we established a relationship between cell number and colony size for strains of Saccharomyces cerevisiae robotically pinned onto solid agar plates in a high-density format. This was used to measure growth rates and estimate relative fitness differences between evolved strains and their ancestors. After controlling for edge effects through both normalization and agar-trimming, we found that colony size is a sensitive measure of fitn

SUBMITTER: Miller JH 

PROVIDER: S-EPMC9560512 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature