The choice of the objective function in flux balance analysis is crucial for predicting replicative lifespans in yeast.

ABSTRACT: Flux balance analysis (FBA) is a powerful tool to study genome-scale models of the cellular metabolism, based on finding the optimal flux distributions over the network. While the objective function is crucial for the outcome, its choice, even though motivated by evolutionary arguments, has not been directly connected to related measures. Here, we used an available multi-scale mathematical model of yeast replicative ageing, integrating cellular metabolism, nutrient sensing and damage accumulation, to systematically test the effect of commonly used objective functions on features of replicative ageing in budding yeast, such as the number of cell divisions and the corresponding time between divisions. The simulations confirmed that assuming maximal growth is essential for reaching realistic

SUBMITTER: Schnitzer B 

PROVIDER: S-EPMC9560524 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature