Tumor-Infiltrating T Cells Can Be Expanded Successfully from Primary Uveal Melanoma after Separation from Their Tumor Environment.

ABSTRACT:

Purpose

To evaluate whether expanded tumor-infiltrating lymphocytes (TILs) can be obtained from primary uveal melanoma (UM) for potential use as adjuvant treatment in patients at risk of developing metastatic disease.

Design

Experimental research study.

Participants

Freshly obtained primary UM from 30 patients.

Methods

Three different methods were used to expand TILs: (1) direct culture from small fragments of fresh tumor tissue, (2) single-cell tissue preparation by enzymatic digestion and subsequent enrichment of mononuclear cells, and (3) selection of CD3⁺ T cells using magnetic beads. Surface expression of costimulatory and inhibitory T-cell markers and T-cell reactivity against autologous tumor cells was assessed. Clinical, histopathologic, gene

SUBMITTER: Gezgin G 

PROVIDER: S-EPMC9560540 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature