Cotransplantation of Limbal Epithelial and Stromal Cells for Ocular Surface Reconstruction.

ABSTRACT:

Purpose

To propose an improved stem cell-based strategy for limbal stem cell deficiency (LSCD) treatment.

Design

Experimental randomized or parallel-group animal study.

Subjects

Fifty adult male New Zealand white rabbits.

Methods

Human limbal stem/progenitor cells (LSCs) and limbal stromal stem/progenitor cells (LSSCs) were cultured in serum-free conditions and further differentiated into corneal epithelial cells and keratocytes, respectively. All cell types were characterized with lineage-specific markers. Gene expression analysis was performed to identify the potential function of LSSCs in corneal regeneration. Two LSCD models of rabbits for transplantations were used: transplantation performed at the time of limbal and corneal epithelial excision (LSCD model

SUBMITTER: Zhu L 

PROVIDER: S-EPMC9560570 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature