Spatial and Transcriptomic Analysis of Perineural Invasion in Oral Cancer.

ABSTRACT:

Purpose

Perineural invasion (PNI), a common occurrence in oral squamous cell carcinomas, is associated with poor survival. Consequently, these tumors are treated aggressively. However, diagnostic criteria of PNI vary and its role as an independent predictor of prognosis has not been established. To address these knowledge gaps, we investigated spatial and transcriptomic profiles of PNI-positive and PNI-negative nerves.

Experimental design

Tissue sections from 142 patients were stained with S100 and cytokeratin antibodies. Nerves were identified in two distinct areas: tumor bulk and margin. Nerve diameter and nerve-to-tumor distance were assessed; survival analyses were performed. Spatial transcriptomic analysis of nerves at varying distances from tumor was performed with Nan

SUBMITTER: Schmitd LB 

PROVIDER: S-EPMC9560986 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature